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Home > Product > ELISA Kit > Human Prostaglandin Reductase 1, PTGR1 ELISA Kit

Human Prostaglandin Reductase 1, PTGR1 ELISA Kit

Human Prostaglandin Reductase 1, PTGR1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.096ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prostaglandin Reductase 1

Code

E1816Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.096ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX) (PubMed: 25619643). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15-oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15-oxo-PGF2-alpha (PubMed: 25619643). Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (PubMed: 25619643) (By similarity). Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity). May play a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (PubMed: 25619643). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity).

UniProt accession

MASS(Da)

35,870

GeneID

22949

Synonyms

15-oxoprostaglandin 13-reductase;D3T-inducible gene 1 protein;DIG-1;Dithiolethione-inducible gene 1 protein;Leukotriene B4 12-hydroxydehydrogenase;NAD(PH-dependent alkenal/one oxidoreductase;PRG-1;Prostaglandin reductase 1;PTGR 1;PTGR1

Gene names

PTGR1

Research area

Immunology

Target protein

PTGR1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1816Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 2.35 2.374 2.303
2.397
16ng/ml 1.443 1.421 1.35
1.398
8ng/ml 0.809 0.848 0.777
0.888
4ng/ml 0.509 0.504 0.433
0.498
2ng/ml 0.256 0.25 0.179
0.244
0ng/ml 0.071 0.071 0
0.071

Precision

We measured random samples of E1816Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 9.5 0.43 4.6
Intra-Assay 2 18 14 0.51 3.6
Intra-Assay 3 18 3 0.21 6.7

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E1816Hu

    $320.00

  • 96T

    E1816Hu

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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