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Home > Product > ELISA Kit > Human Podocalyxin, PCX ELISA Kit

Human Podocalyxin, PCX ELISA Kit

Human Podocalyxin, PCX ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Podocalyxin

Code

E1834Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

UniProt accession

MASS(Da)

58,635

GeneID

5420

Synonyms

GCTM-2 antigen;Gp200;PC;PCLP-1;Podocalyxin;Podocalyxin-like protein 1;PODXL

Gene names

PODXL

Research area

Others

Target protein

PODXL

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1834Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.205 2.138 2.096
2.071
4ng/ml 1.427 1.388 1.345
1.349
2ng/ml 0.878 0.826 0.783
0.773
1ng/ml 0.468 0.455 0.413
0.442
0.5ng/ml 0.222 0.224 0.181
0.225
0ng/ml 0.04 0.042 0
0.044

Precision

We measured random samples of E1834Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.3 0.06 4.8
Intra-Assay 2 18 3.4 0.24 7.1
Intra-Assay 3 18 4.4 0.17 3.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E1834Hu

    $320.00

  • 96T

    E1834Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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