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Home > Product > ELISA Kit > Human Peroxisome Proliferative Activated Receptor Gama Coactivator 1 Alpha, PPARGC1 ELISA Kit

Human Peroxisome Proliferative Activated Receptor Gama Coactivator 1 Alpha, PPARGC1 ELISA Kit

Human Peroxisome Proliferative Activated Receptor Gama Coactivator 1 Alpha, PPARGC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.097pg/ml

Detection range: 0.1-40pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.097pg/ml

Detection range: 0.1-40pg/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Peroxisome Proliferative Activated Receptor Gama Coactivator 1 Alpha

Code

E1876Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.097pg/ml

Detection range

0.1-40pg/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.

UniProt accession

MASS(Da)

91,027

GeneID

10891

Synonyms

Ligand effect modulator 6;Peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC-1-alpha;PPAR-gamma coactivator 1-alpha;PPARGC 1A;PPARGC1A;PPARGC-1-alpha

Gene names

PPARGC1A

Research area

Metabolism

Target protein

PPARGC1A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1876Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24pg/ml 2.152 2.135 2.075
2.118
12pg/ml 1.444 1.446 1.387
1.448
6pg/ml 0.806 0.836 0.777
0.867
3pg/ml 0.475 0.472 0.413
0.47
1.5pg/ml 0.297 0.303 0.244
0.31
0pg/ml 0.066 0.06 0
0.053

Precision

We measured random samples of E1876Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.9 0.21 4.3
Intra-Assay 2 18 9.2 0.53 5.8
Intra-Assay 3 18 13.5 0.6 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E1876Hu

    $320.00

  • 96T

    E1876Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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