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Home > Product > ELISA Kit > Human Beclin-1, BECN1 ELISA Kit

Human Beclin-1, BECN1 ELISA Kit

Human Beclin-1, BECN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.1ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.1ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Beclin-1

Code

E2011Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.1ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays a central role in autophagy (PubMed: 23184933, PubMed: 28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed: 20643123, PubMed: 20208530, PubMed: 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed: 25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed: 9765397). May play a role in antiviral host defense. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.

UniProt accession

MASS(Da)

51,896

GeneID

8678

Synonyms

Beclin-1;Beclin-1-C 35 kDa;Beclin-1-C 37 kDa;BECN 1;BECN1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197

Gene names

BECN1

Research area

Others

Target protein

BECN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2011Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
32ng/ml 1.874 1.79 1.741
1.707
16ng/ml 1.124 0.994 0.945
0.865
8ng/ml 0.639 0.577 0.528
0.515
4ng/ml 0.138 0.168 0.119
0.198
2ng/ml 0.102 0.108 0.058
0.114
0ng/ml 0.058 0.049 0
0.04

Precision

We measured random samples of E2011Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 18.1 0.88 4.8
Intra-Assay 2 18 26.9 1.06 3.9
Intra-Assay 3 18 9.3 0.38 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2011Hu

    $320.00

  • 96T

    E2011Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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