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Home > Product > ELISA Kit > Human Histone Deacetylase 2, HDAC2 ELISA Kit

Human Histone Deacetylase 2, HDAC2 ELISA Kit

Human Histone Deacetylase 2, HDAC2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.031ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Histone Deacetylase 2

Code

E2025Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.031ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A.

UniProt accession

MASS(Da)

55,364

GeneID

3066

Synonyms

HD2;HDAC 2;HDAC2;Histone deacetylase 2

Gene names

HDAC2

Research area

Others

Target protein

HDAC2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2025Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.289 2.31 2.257
2.33
8ng/ml 1.539 1.535 1.482
1.531
4ng/ml 0.818 0.772 0.72
0.726
2ng/ml 0.55 0.501 0.448
0.452
1ng/ml 0.313 0.272 0.219
0.23
0ng/ml 0.037 0.053 0
0.068

Precision

We measured random samples of E2025Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.6 0.13 3.6
Intra-Assay 2 18 7.9 0.22 2.8
Intra-Assay 3 18 2.5 0.13 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2025Hu

    $320.00

  • 96T

    E2025Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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