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Home > Product > ELISA Kit > Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

Human Interferon Regulatory Factor 3, IRF3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interferon Regulatory Factor 3

Code

E2069Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.054ng/ml

Detection range

0.1-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters (PubMed: 11846977, PubMed: 16846591, PubMed: 16979567, PubMed: 20049431, PubMed: 32972995). Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction (PubMed: 16846591, PubMed: 16979567, PubMed: 20049431). Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases (PubMed: 22394562, PubMed: 25636800, PubMed: 27302953). This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes (PubMed: 16154084, PubMed: 27302953, PubMed: 33440148). Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages (PubMed: 16846591). In response to Sendai virus infection, is recruited by TOMM70:HSP90AA1 to mitochondrion and forms an apoptosis complex TOMM70:HSP90AA1:IRF3:BAX inducing apoptosis (PubMed: 25609812). Key transcription factor regulating the IFN response during SARS-CoV-2 infection (PubMed: 33440148).

UniProt accession

MASS(Da)

47,219

GeneID

3661

Synonyms

Interferon regulatory factor 3;IRF 3;IRF3;IRF-3

Gene names

IRF3

Research area

Others

Target protein

IRF3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2069Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.068 2.063 2.002
2.057
8ng/ml 1.33 1.323 1.263
1.317
4ng/ml 0.937 0.916 0.856
0.895
2ng/ml 0.396 0.465 0.405
0.534
1ng/ml 0.278 0.274 0.213
0.269
0ng/ml 0.049 0.06 0
0.072

Precision

We measured random samples of E2069Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2069Hu

    $320.00

  • 96T

    E2069Hu

    $458.00

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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