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Home > Product > ELISA Kit > Human Semaphorin 3A, SEMA3A ELISA Kit

Human Semaphorin 3A, SEMA3A ELISA Kit

Human Semaphorin 3A, SEMA3A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.024ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Semaphorin 3A

Code

E2078Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.024ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.

UniProt accession

MASS(Da)

88,889

GeneID

10371

Synonyms

Sema III;SEMA3A;Semaphorin III;Semaphorin-3A

Gene names

SEMA3A

Research area

Cardiovascular

Target protein

SEMA3A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2078Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.125 2.136 2.093
2.147
6ng/ml 1.897 1.862 1.819
1.828
3ng/ml 1.186 1.19 1.147
1.194
1.5ng/ml 0.626 0.673 0.63
0.72
0.75ng/ml 0.423 0.416 0.373
0.409
0ng/ml 0.049 0.043 0
0.038

Precision

We measured random samples of E2078Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.5 0.46 7
Intra-Assay 2 18 1.4 0.08 5.4
Intra-Assay 3 18 2.3 0.09 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2078Hu

    $320.00

  • 96T

    E2078Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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