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Home > Product > ELISA Kit > Human Leukocyte Elastase Inhibitor, LEI ELISA Kit

Human Leukocyte Elastase Inhibitor, LEI ELISA Kit

Human Leukocyte Elastase Inhibitor, LEI ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.057ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.057ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Leukocyte Elastase Inhibitor

Code

E2231Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.057ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Neutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis (PubMed: 30692621). Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3 (PubMed: 11747453, PubMed: 30692621). Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity (PubMed: 23269243). During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed: 30692621). When secreted, promotes the proliferation of beta-cells via its protease inhibitory function (PubMed: 26701651).

UniProt accession

MASS(Da)

42,742

GeneID

1992

Synonyms

EI;LEI;Leukocyte elastase inhibitor;M/NEI;Monocyte/neutrophil elastase inhibitor;Peptidase inhibitor 2;PI-2;Serpin B1;SERPINB 1;SERPINB1

Gene names

SERPINB1

Research area

Cell Biology

Target protein

SERPINB1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2231Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.355 2.278 2.228
2.202
12ng/ml 1.743 1.617 1.567
1.492
6ng/ml 1.126 1.129 1.079
1.132
3ng/ml 0.758 0.694 0.644
0.631
1.5ng/ml 0.47 0.462 0.412
0.454
0ng/ml 0.05 0.051 0
0.051

Precision

We measured random samples of E2231Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2231Hu

    $320.00

  • 96T

    E2231Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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