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Home > Product > ELISA Kit > Human Glycyl Trna Synthetase, GARS ELISA Kit

Human Glycyl Trna Synthetase, GARS ELISA Kit

Human Glycyl Trna Synthetase, GARS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.03ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.03ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glycyl Trna Synthetase

Code

E2239Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.03ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acyl-CoA synthetase that catalyzes the activation of bile acids via formation of bile acid CoA thioesters which is necessary for their subsequent conjugation with glycine or taurine (PubMed: 107$498.00|$360.0048, PubMed: 11980911). Both primary bile acids (cholic acid and chenodeoxycholic acid) and secondary bile acids (deoxycholic acid and lithocholic acid) are the principal substrates (PubMed: 107$498.00|$360.0048, PubMed: 11980911). Also exhibits acyl CoA synthetase activity that activates very long-chain fatty acids (VLCFAs) by catalyzing the formation of fatty acyl-CoA (PubMed: 10479480). In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol (PubMed: 11980911). Exhibits long-chain fatty acids (LCFA) transport activity (PubMed: 20530735). Plays an important role in hepatic fatty acid uptake and bile acid reconjugation and recycling but not in de novo synthesis of bile acids (By similarity).

UniProt accession

MASS(Da)

75,385

GeneID

10998

Synonyms

BA-CoA ligase;BACS;BAL;Bile acid-CoA ligase;Bile acyl-CoA synthetase;Cholate--CoA ligase;FATP-5;Fatty acid transport protein 5;Fatty-acid-coenzyme A ligase, very long-chain 3;SLC27A5;Solute carrier family 27 member 5;Very long-chain acyl-CoA synthetase homolog 2;Very long-chain acyl-CoA synthetase-related protein;VLACSR;VLACS-related;VLCSH2;VLCS-H2

Gene names

SLC27A5

Research area

Cardiovascular

Target protein

SLC27A5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2239Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.35 2.331 2.304
2.311
1200ng/L 1.217 1.311 1.285
1.405
600ng/L 0.75 0.734 0.707
0.717
300ng/L 0.413 0.4 0.374
0.388
150ng/L 0.142 0.148 0.122
0.154
0ng/L 0.034 0.026 0
0.019

Precision

We measured random samples of E2239Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 318.9 14.68 4.6
Intra-Assay 2 18 1046.8 43.52 4.2
Intra-Assay 3 18 1228.5 35.66 2.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E2239Hu

    $320.00

  • 96T

    E2239Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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