Cytochrome P450 1A2

E2281Hu

Sandwich

48T,96T

5.15ng/L

10-2000ng/L

Serum, plasma, cell culture supernates

Human

2-8ºC

1h 30m

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 9435160, PubMed: 10681376, PubMed: 11555828, PubMed: 12865317, PubMed: 19965576). Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed: 11555828, PubMed: 12865317). Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed: 11555828, PubMed: 12865317). Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed: 21576599). May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R,S) stereoisomer (PubMed: 19965576). Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed: 9435160). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed: 21068195). Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed: 14725854). Metabolizes caffeine via N3-demethylation (Probable).

58,407

1544

Cholesterol 25-hydroxylase;CYP1A2;CYPIA2;Cytochrome P(3450;Cytochrome P450 1A2;Cytochrome P450 4;Cytochrome P450-P3;Hydroperoxy icosatetraenoate dehydratase

CYP1A2

Others

CYP1A2