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Home > Product > ELISA Kit > Human Furin, FUR ELISA Kit

Human Furin, FUR ELISA Kit

Human Furin, FUR ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 6.93ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 6.93ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Furin

Code

E2321Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

6.93ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed: 11799113, PubMed: 1629222, PubMed: 1713771, PubMed: 2251280, PubMed: 24666235, PubMed: 25974265, PubMed: 7592877, PubMed: 7690548, PubMed: 9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed: 7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed: 20489134, PubMed: 21763278). (Microbial infection) Probably cleaves and activates anthrax and diphtheria toxins. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.

UniProt accession

MASS(Da)

86,678

GeneID

5045

Synonyms

Dibasic-processing enzyme;FURIN;PACE;Paired basic amino acid residue-cleaving enzyme

Gene names

FURIN

Research area

Cell Biology

Target protein

FURIN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2321Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.132 2.209 2.154
2.286
800ng/L 1.404 1.395 1.34
1.386
400ng/L 0.922 0.873 0.818
0.824
200ng/L 0.529 0.512 0.457
0.495
100ng/L 0.296 0.303 0.249
0.311
0ng/L 0.051 0.055 0
0.058

Precision

We measured random samples of E2321Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 286.8 19.51 6.8
Intra-Assay 2 18 538.4 33.5 6.2
Intra-Assay 3 18 1070.4 43.46 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2321Hu

    $320.00

  • 96T

    E2321Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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