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Home > Product > ELISA Kit > Human Frizzled-2, FZD2 ELISA Kit

Human Frizzled-2, FZD2 ELISA Kit

Human Frizzled-2, FZD2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.013ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.013ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Frizzled-2

Code

E2369Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.013ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (PubMed: 25759469). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. (Microbial infection) Acts as a receptor for C.difficile toxin TcdB in the colonic epithelium (PubMed: 27680706, PubMed: 29748286). TcdB occupies the binding site for Wnt-adducted palmitoleate in frizzled receptors and TcdB-binding prevents Wnt-binding and downstream Wnt signaling (PubMed: 29748286).

UniProt accession

MASS(Da)

63,554

GeneID

2535

Synonyms

Frizzled-2;Fz-2;FZD 2;FZD2;FzE2;hFz2

Gene names

FZD2

Research area

Signal Transduction

Target protein

FZD2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2369Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 1.989 1.98 1.933
1.971
3.2ng/ml 1.023 1.001 0.954
0.979
1.6ng/ml 0.407 0.453 0.406
0.499
0.8ng/ml 0.201 0.229 0.182
0.257
0.4ng/ml 0.16 0.14 0.092
0.12
0ng/ml 0.038 0.047 0
0.056

Precision

We measured random samples of E2369Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.3 0.15 2.8
Intra-Assay 2 18 2.5 0.11 4.3
Intra-Assay 3 18 2.9 0.1 3.4

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2369Hu

    $320.00

  • 96T

    E2369Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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