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Home > Product > ELISA Kit > Human Methionyl Aminopeptidase 2, METAP2 ELISA Kit

Human Methionyl Aminopeptidase 2, METAP2 ELISA Kit

Human Methionyl Aminopeptidase 2, METAP2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Methionyl Aminopeptidase 2

Code

E2382Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val Peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.

UniProt accession

MASS(Da)

52,892

GeneID

10988

Synonyms

Initiation factor 2-associated 67 kDa glycoprotein;MAP 2;METAP 2;METAP2;Methionine aminopeptidase 2;p67;p67eIF2;Peptidase M

Gene names

METAP2

Research area

Signal Transduction

Target protein

METAP2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2382Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.127 2.193 2.145
2.258
3.2ng/ml 1.612 1.571 1.523
1.53
1.6ng/ml 1 1.002 0.954
1.003
0.8ng/ml 0.737 0.732 0.684
0.726
0.4ng/ml 0.397 0.434 0.386
0.472
0ng/ml 0.035 0.048 0
0.061

Precision

We measured random samples of E2382Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E2382Hu

    $320.00

  • 96T

    E2382Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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