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Home > Product > ELISA Kit > Human Replication Initiator 1, REPIN1 ELISA Kit

Human Replication Initiator 1, REPIN1 ELISA Kit

Human Replication Initiator 1, REPIN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.48ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Replication Initiator 1

Code

E2401Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.48ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending.

UniProt accession

MASS(Da)

63,575

GeneID

29803

Synonyms

60 kDa origin-specific DNA-binding protein;60 kDa replication initiation region protein;ATT-binding protein;DHFR oribeta-binding protein RIP60;REPIN 1;REPIN1;Replication initiator 1;Zinc finger protein 464

Gene names

REPIN1

Research area

Others

Target protein

REPIN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2401Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 1.995 1.985 1.946
1.975
1200ng/L 1.112 0.997 0.958
0.882
600ng/L 0.604 0.587 0.547
0.569
300ng/L 0.155 0.166 0.127
0.176
150ng/L 0.08 0.108 0.069
0.136
0ng/L 0.027 0.039 0
0.051

Precision

We measured random samples of E2401Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1649.9 48.98 3
Intra-Assay 2 18 515.9 20.09 3.9
Intra-Assay 3 18 902.6 32.9 3.6

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2401Hu

    $320.00

  • 96T

    E2401Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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