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Home > Product > ELISA Kit > Human Ribonuclease 3, RNASEN ELISA Kit

Human Ribonuclease 3, RNASEN ELISA Kit

Human Ribonuclease 3, RNASEN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ribonuclease 3

Code

E2404Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.

UniProt accession

MASS(Da)

159,316

GeneID

29102

Synonyms

DROSHA;p241;Protein Drosha;Ribonuclease 3;Ribonuclease III;RNase III

Gene names

DROSHA

Research area

Epigenetics and Nuclear Signaling

Target protein

DROSHA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2404Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 2.304 2.265 2.217
2.227
4ng/ml 1.701 1.817 1.769
1.933
2ng/ml 1.16 1.23 1.181
1.299
1ng/ml 0.722 0.678 0.629
0.633
0.5ng/ml 0.362 0.375 0.326
0.388
0ng/ml 0.065 0.048 0
0.032

Precision

We measured random samples of E2404Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.12 6.8
Intra-Assay 2 18 2.5 0.1 4.1
Intra-Assay 3 18 0.8 0.05 6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2404Hu

    $320.00

  • 96T

    E2404Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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