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Home > Product > ELISA Kit > Human Rala Binding Protein 1, RALBP1 ELISA Kit

Human Rala Binding Protein 1, RALBP1 ELISA Kit

Human Rala Binding Protein 1, RALBP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rala Binding Protein 1

Code

E2409Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.03ng/ml

Detection range

0.05-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.

UniProt accession

MASS(Da)

76,063

GeneID

10928

Synonyms

76 kDa Ral-interacting protein;Dinitrophenyl S-glutathione ATPase;DNP-SG ATPase;RalA-binding protein 1;RALBP 1;RALBP1;Ral-interacting protein 1

Gene names

RALBP1

Research area

Cell Biology

Target protein

RALBP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2409Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.161 2.29 2.253
2.419
12ng/ml 1.397 1.414 1.377
1.432
6ng/ml 0.733 0.675 0.638
0.617
3ng/ml 0.399 0.355 0.318
0.311
1.5ng/ml 0.13 0.145 0.108
0.159
0ng/ml 0.041 0.037 0
0.033

Precision

We measured random samples of E2409Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.1 0.27 4.4
Intra-Assay 2 18 8.6 0.41 4.7
Intra-Assay 3 18 15.1 0.47 3.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2409Hu

    $320.00

  • 96T

    E2409Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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