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Home > Product > ELISA Kit > Human Dolichol Kinase, DOLK ELISA Kit

Human Dolichol Kinase, DOLK ELISA Kit

Human Dolichol Kinase, DOLK ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dolichol Kinase

Code

E2411Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors.

UniProt accession

MASS(Da)

59,268

GeneID

22845

Synonyms

Dolichol kinase;DOLK;Transmembrane protein 15

Gene names

DOLK

Research area

Others

Target protein

DOLK

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2411Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 1.879 1.771 1.745
1.662
4ng/ml 1.054 0.985 0.96
0.917
2ng/ml 0.523 0.576 0.551
0.63
1ng/ml 0.167 0.154 0.129
0.142
0.5ng/ml 0.099 0.081 0.055
0.062
0ng/ml 0.027 0.025 0
0.023

Precision

We measured random samples of E2411Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.2 0.27 3.3
Intra-Assay 2 18 4.8 0.3 6.2
Intra-Assay 3 18 2.2 0.1 4.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2411Hu

    $320.00

  • 96T

    E2411Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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