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Home > Product > ELISA Kit > Human Opsin 5, OPN5 ELISA Kit

Human Opsin 5, OPN5 ELISA Kit

Human Opsin 5, OPN5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.07ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Opsin 5

Code

E2414Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.07ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

G-protein coupled receptor which selectively activates G(i) type G proteins via ultraviolet A (UVA) light-mediated activation in the retina (By similarity). Preferentially binds the chromophore 11-cis retinal and is a bistable protein that displays emission peaks at 380 nm (UVA light) and 470 nm (blue light) (PubMed: 22043319). Required for the light-response in the inner plexiform layer, and contributes to the regulation of the light-response in the nerve fiber layer, via phosphorylated DAT/SLC6A3 dopamine uptake (By similarity). Involved in local corneal and retinal circadian rhythm photoentrainment via modulation of the UVA light-induced phase-shift of the retina clock (By similarity). Acts as a circadian photoreceptor in the outer ear, via modulation of circadian clock-gene expression in response to violet light during the light-to-dark transition phase and night phase of the circadian cycle (By similarity). Required in the retina to negatively regulate hyaloid vessel regression during postnatal development via light-dependent OPN5-SLC32A1-DRD2-VEGFR2 signaling (By similarity). Involved in the light-dependent regulation of retina and vitreous compartment dopamine levels (By similarity).

UniProt accession

MASS(Da)

39,727

GeneID

221391

Synonyms

G-protein coupled receptor 136;G-protein coupled receptor PGR12;Neuropsin;OPN 5;OPN5;Opsin-5;Transmembrane protein 13

Gene names

OPN5

Research area

Neuroscience; Cardiovascular

Target protein

OPN5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2414Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 1.967 2.1 2.058
2.233
20ng/ml 1.499 1.401 1.359
1.303
10ng/ml 0.777 0.659 0.618
0.542
5ng/ml 0.444 0.379 0.337
0.313
2.5ng/ml 0.128 0.135 0.094
0.143
0ng/ml 0.027 0.042 0
0.056

Precision

We measured random samples of E2414Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 10.3 0.46 4.5
Intra-Assay 2 18 16.5 0.83 5
Intra-Assay 3 18 22.2 0.71 3.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2414Hu

    $320.00

  • 96T

    E2414Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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