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Home > Product > ELISA Kit > Human Proteasome Subunit Beta Type 6, PSMB6 ELISA Kit

Human Proteasome Subunit Beta Type 6, PSMB6 ELISA Kit

Human Proteasome Subunit Beta Type 6, PSMB6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.98ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.98ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Proteasome Subunit Beta Type 6

Code

E2427Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.98ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic Peptides (20S-PA28 complex). Within the 20S core complex, PSMB6 displays a peptidylglutamyl-hydrolizing activity also termed postacidic or caspase-like activity, meaning that the Peptides bond hydrolysis occurs directly after acidic residues.

UniProt accession

MASS(Da)

25,358

GeneID

5694

Synonyms

Macropain delta chain;Multicatalytic endopeptidase complex delta chain;Proteasome delta chain;Proteasome subunit beta type-6;Proteasome subunit Y;PSMB 6;PSMB6

Gene names

PSMB6

Research area

Others

Target protein

PSMB6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2427Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.072 2.132 2.099
2.192
400ng/L 1.205 1.292 1.258
1.379
200ng/L 0.802 0.754 0.721
0.706
100ng/L 0.499 0.476 0.443
0.454
50ng/L 0.248 0.235 0.202
0.223
0ng/L 0.026 0.033 0
0.04

Precision

We measured random samples of E2427Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 81.7 4.84 5.9
Intra-Assay 2 18 454.8 15.65 3.4
Intra-Assay 3 18 248.7 12.47 5

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2427Hu

    $320.00

  • 96T

    E2427Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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