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Home > Product > ELISA Kit > Human Cytochrome P450 11B2, Mitochondrial, ALDOS ELISA Kit

Human Cytochrome P450 11B2, Mitochondrial, ALDOS ELISA Kit

Human Cytochrome P450 11B2, Mitochondrial, ALDOS ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 10.08ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 10.08ng/L

Detection range: 20-6000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 11B2, Mitochondrial

Code

E2440Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

10.08ng/L

Detection range

20-6000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase that catalyzes the biosynthesis of adrenal mineralocorticoid aldosterone (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone/21-hydroxyprogesterone, namely 11-beta hydroxylation followed with two successive oxidations at C18 to yield 18-hydroxy and then 18-aldehyde derivatives, resulting in the formation of aldosterone (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed: 11856349, PubMed: 23322723, PubMed: 1594605, PubMed: 9814506).

UniProt accession

MASS(Da)

57,560

GeneID

1585

Synonyms

ALDOS;Aldosterone Synthase;Aldosterone-synthesizing enzyme;Corticosterone 18-monooxygenase, CYP11B2;CYP11B2;CYPXIB2;Cytochrome P450 11B2, mitochondrial;Cytochrome P-450Aldo;Cytochrome P-450C18;Steroid 11-beta-hydroxylase, CYP11B2;Steroid 18-hydroxylase

Gene names

CYP11B2

Research area

Others

Target protein

CYP11B2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2440Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3200ng/L 2.324 2.368 2.342
2.412
1600ng/L 1.432 1.334 1.308
1.236
800ng/L 0.623 0.614 0.588
0.605
400ng/L 0.425 0.424 0.398
0.422
200ng/L 0.136 0.127 0.101
0.118
0ng/L 0.015 0.026 0
0.037

Precision

We measured random samples of E2440Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 544.8 24.02 4.4
Intra-Assay 2 18 1949.7 64.69 3.3
Intra-Assay 3 18 1399 72.64 5.2

Protocols

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  • Size

    Code

    Price

  • 48T

    E2440Hu

    $320.00

  • 96T

    E2440Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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