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Home > Product > ELISA Kit > Human Ephrin Type A Receptor 3, EPHA3 ELISA Kit

Human Ephrin Type A Receptor 3, EPHA3 ELISA Kit

Human Ephrin Type A Receptor 3, EPHA3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.019ng/ml

Detection range: 0.05-15ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ephrin Type A Receptor 3

Code

E2444Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.019ng/ml

Detection range

0.05-15ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

UniProt accession

MASS(Da)

110,131

GeneID

2042

Synonyms

EK4;EPHA 3;EPHA3;EPH-like kinase 4;Eph-like tyrosine kinase 1;Ephrin type-A receptor 3;HEK;hEK4;Human embryo kinase;Tyrosine-protein kinase receptor ETK1;Tyrosine-protein kinase TYRO4

Gene names

EPHA3

Research area

Others

Target protein

EPHA3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2444Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
8ng/ml 1.999 1.961 1.91
1.924
4ng/ml 0.977 0.953 0.902
0.929
2ng/ml 0.466 0.468 0.417
0.47
1ng/ml 0.223 0.217 0.166
0.21
0.5ng/ml 0.073 0.094 0.043
0.115
0ng/ml 0.052 0.051 0
0.05

Precision

We measured random samples of E2444Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.2 0.16 3.8
Intra-Assay 2 18 6.1 0.15 2.5
Intra-Assay 3 18 3.1 0.11 3.6

Protocols

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  • Size

    Code

    Price

  • 48T

    E2444Hu

    $320.00

  • 96T

    E2444Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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