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Home > Product > ELISA Kit > Human Adenylate Cyclase Type 10, ADCY10 ELISA Kit

Human Adenylate Cyclase Type 10, ADCY10 ELISA Kit

Human Adenylate Cyclase Type 10, ADCY10 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Adenylate Cyclase Type 10

Code

E2460Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Catalyzes the formation of the signaling molecule cAMP (PubMed: 12609998, PubMed: 15659711, PubMed: 24616449, PubMed: 25040695, PubMed: 24567411). May function as sensor that mediates responses to changes in cellular bicarbonate and CO(2) levels (PubMed: 15659711, PubMed: 17591988). Has a critical role in mammalian spermatogenesis by producing the cAMP which regulates cAMP-responsive nuclear factors indispensable for sperm maturation in the epididymis. Induces capacitation, the maturational process that sperm undergo prior to fertilization (By similarity). Involved in ciliary beat regulation (PubMed: 17591988).

UniProt accession

MASS(Da)

187,149

GeneID

55811

Synonyms

ADCY 10;ADCY10;Adenylate cyclase homolog;Adenylate cyclase type 10;AH-related protein;Germ cell soluble adenylyl cyclase;hsAC;sAC;Testicular soluble adenylyl cyclase;

Gene names

ADCY10

Research area

Signal Transduction

Target protein

ADCY10

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2460Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.202 2.31 2.241
2.419
8ng/ml 1.229 1.314 1.245
1.399
4ng/ml 0.771 0.754 0.685
0.737
2ng/ml 0.418 0.45 0.381
0.481
1ng/ml 0.327 0.32 0.251
0.312
0ng/ml 0.066 0.069 0
0.072

Precision

We measured random samples of E2460Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2.4 0.15 6.3
Intra-Assay 2 18 10.3 0.37 3.6
Intra-Assay 3 18 5.7 0.25 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2460Hu

    $320.00

  • 96T

    E2460Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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