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Home > Product > ELISA Kit > Human Thyroxine 5-Deiodinase, DIO3 ELISA Kit

Human Thyroxine 5-Deiodinase, DIO3 ELISA Kit

Human Thyroxine 5-Deiodinase, DIO3 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.098ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.098ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Thyroxine 5-Deiodinase

Code

E2462Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.098ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.

UniProt accession

MASS(Da)

33,947

GeneID

1735

Synonyms

5DIII;DIO 3;DIO3;DIOIII;Thyroxine 5-deiodinase;Type 3 DI;Type III iodothyronine deiodinase

Gene names

DIO3

Research area

Others

Target protein

DIO3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2462Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.145 2.048 1.994
1.951
20ng/ml 1.471 1.462 1.408
1.453
10ng/ml 0.735 0.697 0.643
0.659
5ng/ml 0.375 0.405 0.352
0.436
2.5ng/ml 0.183 0.196 0.142
0.209
0ng/ml 0.055 0.054 0
0.052

Precision

We measured random samples of E2462Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 23.8 1.04 4.4
Intra-Assay 2 18 10.7 0.51 4.8
Intra-Assay 3 18 7.6 0.44 5.7

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E2462Hu

    $320.00

  • 96T

    E2462Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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