Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Background
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2490Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
40ng/ml | 1.962 | 1.843 | 1.813 |
1.724 | |||
20ng/ml | 1.089 | 1.104 | 1.074 |
1.119 | |||
10ng/ml | 0.606 | 0.514 | 0.483 |
0.421 | |||
5ng/ml | 0.252 | 0.204 | 0.174 |
0.155 | |||
2.5ng/ml | 0.086 | 0.096 | 0.066 |
0.106 | |||
0ng/ml | 0.033 | 0.03 | 0 |
0.027 |
Precision
We measured random samples of E2490Hu within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 12 | 0.52 | 4.3 |
Intra-Assay | 2 | 18 | 14.5 | 0.54 | 3.7 |
Intra-Assay | 3 | 18 | 31.3 | 1.15 | 3.7 |
Protocols
Customer reviews and Q&As
Associated products
Human Microtubule-associated Proteins 1A/1B Light Chain 3B, MAP1LC3B ELISA Kit
Human Microtubule-associated Proteins 1A, 1B Light Chain 3A, MAPILC3A ELISA Kit
Mouse Microtubule-associated Protein 1 Light Chain 3 Alpha, MAP1LC3A ELISA Kit
Mouse Microtubule-associated Proteins 1A/1B Light Chain 3B, MAP1LC3B ELISA Kit