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Home > Product > ELISA Kit > Human Semaphorin 7A, SEMA7A ELISA Kit

Human Semaphorin 7A, SEMA7A ELISA Kit

Human Semaphorin 7A, SEMA7A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.046ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Semaphorin 7A

Code

E2538Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.046ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.

UniProt accession

MASS(Da)

74,824

GeneID

8482

Synonyms

CD antigen CD108;CDw108;JMH blood group antigen;John-Milton-Hargen human blood group Ag;Sema K1;Sema L;SEMA7A;Semaphorin-7A;Semaphorin-K1;Semaphorin-L

Gene names

SEMA7A

Research area

Neuroscience

Target protein

SEMA7A

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2538Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.166 2.256 2.203
2.347
12ng/ml 1.188 1.194 1.141
1.2
6ng/ml 0.677 0.746 0.693
0.816
3ng/ml 0.398 0.436 0.383
0.475
1.5ng/ml 0.191 0.194 0.141
0.198
0ng/ml 0.058 0.053 0
0.049

Precision

We measured random samples of E2538Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.2 0.38 5.2
Intra-Assay 2 18 15.3 0.38 2.5
Intra-Assay 3 18 4.9 0.3 6.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2538Hu

    $320.00

  • 96T

    E2538Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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