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Home > Product > ELISA Kit > Human Sorting Nexin 1, SNX1 ELISA Kit

Human Sorting Nexin 1, SNX1 ELISA Kit

Human Sorting Nexin 1, SNX1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Sorting Nexin 1

Code

E2543Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed: 12198132). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity (PubMed: 19816406, PubMed: 23085988). Involved in retrograde endosome-to-TGN transport of lysosomal enzyme receptors (IGF2R, M6PR and SORT1) and Shiginella dysenteria toxin stxB. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi (PubMed: 12198132, PubMed: 15$498.00|$360.00486, PubMed: 17550970, PubMed: 17101778, PubMed: 18088323, PubMed: 21040701). Involvement in retromer-independent endocytic trafficking of P2RY1 and lysosomal degradation of protease-activated receptor-1/F2R (PubMed: 16407403, PubMed: 20070609). Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN (PubMed: 20604901). Required for endocytosis of DRD5 upon agonist stimulation but not for basal receptor trafficking (PubMed: 23152$498.00|$360.00).

UniProt accession

MASS(Da)

59,070

GeneID

6642

Synonyms

SNX 1;SNX1;Sorting nexin-1

Gene names

SNX1

Research area

Signal Transduction

Target protein

SNX1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2543Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 1.72 1.788 1.749
1.857
8ng/ml 0.955 0.993 0.953
1.031
4ng/ml 0.491 0.473 0.434
0.456
2ng/ml 0.203 0.192 0.153
0.181
1ng/ml 0.062 0.071 0.032
0.081
0ng/ml 0.042 0.039 0
0.037

Precision

We measured random samples of E2543Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.8 0.3 4.3
Intra-Assay 2 18 11.6 0.39 3.4
Intra-Assay 3 18 4.2 0.18 4.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2543Hu

    $320.00

  • 96T

    E2543Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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