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Home > Product > ELISA Kit > Human Tnf Receptor Associated Factor 6, TRAF6 ELISA Kit

Human Tnf Receptor Associated Factor 6, TRAF6 ELISA Kit

Human Tnf Receptor Associated Factor 6, TRAF6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.57ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.57ng/L

Detection range: 20-3500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tnf Receptor Associated Factor 6

Code

E2607Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.57ng/L

Detection range

20-3500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.

UniProt accession

MASS(Da)

59,573

GeneID

7189

Synonyms

E3 ubiquitin-protein ligase TRAF6;Interleukin-1 signal transducer;RING finger protein 85;RING-type E3 ubiquitin transferase TRAF6;TNF receptor-associated factor 6;TRAF 6;TRAF6

Gene names

TRAF6

Research area

Signal Transduction

Target protein

TRAF6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2607Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.127 2.204 2.158
2.281
1000ng/L 1.43 1.41 1.365
1.39
500ng/L 0.594 0.604 0.558
0.613
250ng/L 0.291 0.338 0.292
0.385
125ng/L 0.204 0.185 0.139
0.166
0ng/L 0.038 0.045 0
0.053

Precision

We measured random samples of E2607Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1301.5 48.94 3.8
Intra-Assay 2 18 404.4 15.67 3.9
Intra-Assay 3 18 553.3 22.43 4.1

Protocols

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  • Size

    Code

    Price

  • 48T

    E2607Hu

    $320.00

  • 96T

    E2607Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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