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Home > Product > ELISA Kit > Human General Transcription Factor Iih Subunit 1, GTF2H1 ELISA Kit

Human General Transcription Factor Iih Subunit 1, GTF2H1 ELISA Kit

Human General Transcription Factor Iih Subunit 1, GTF2H1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.54ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.54ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

General Transcription Factor Iih Subunit 1

Code

E2711Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.54ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.

UniProt accession

MASS(Da)

62,032

GeneID

2965

Synonyms

Basic transcription factor 2 62 kDa subunit;BTF2 p62;General transcription factor IIH polypeptide 1;General transcription factor IIH subunit 1;GTF2H1;TFIIH basal transcription factor complex p62 subunit

Gene names

GTF2H1

Research area

Epigenetics and Nuclear Signaling

Target protein

GTF2H1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2711Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.281 2.163 2.108
2.045
600ng/L 1.363 1.462 1.406
1.561
300ng/L 0.946 0.873 0.818
0.8
150ng/L 0.501 0.455 0.4
0.409
75ng/L 0.233 0.233 0.178
0.233
0ng/L 0.043 0.055 0
0.067

Precision

We measured random samples of E2711Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 485.2 13.47 2.8
Intra-Assay 2 18 256.6 13.9 5.4
Intra-Assay 3 18 290.5 12.46 4.3

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2711Hu

    $320.00

  • 96T

    E2711Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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