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Home > Product > ELISA Kit > Human Glutaredoxin-related Protein 5, Mitochondrial, GLRX5 ELISA Kit

Human Glutaredoxin-related Protein 5, Mitochondrial, GLRX5 ELISA Kit

Human Glutaredoxin-related Protein 5, Mitochondrial, GLRX5 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 7.08ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 7.08ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Glutaredoxin-Related Protein 5, Mitochondrial

Code

E2717Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

7.08ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed: 20364084, PubMed: 23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed: 20364084, PubMed: 23615440, PubMed: 24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed: 20364084).

UniProt accession

MASS(Da)

16,628

GeneID

51218

Synonyms

GLRX 5;GLRX5;Glutaredoxin-related protein 5, mitochondrial;Monothiol glutaredoxin-5

Gene names

GLRX5

Research area

Signal Transduction

Target protein

GLRX5

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2717Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.488 2.558 2.52
2.628
800ng/L 1.473 1.446 1.408
1.419
400ng/L 0.772 0.747 0.709
0.722
200ng/L 0.519 0.494 0.457
0.47
100ng/L 0.198 0.209 0.172
0.221
0ng/L 0.039 0.038 0
0.037

Precision

We measured random samples of E2717Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 974 31.71 3.3
Intra-Assay 2 18 359.2 21.88 6.1
Intra-Assay 3 18 571.7 28.52 5

Protocols

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  • Size

    Code

    Price

  • 48T

    E2717Hu

    $320.00

  • 96T

    E2717Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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