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Home > Product > ELISA Kit > Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

Human Interferon Regulatory Factor 9, IRF9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.58ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.58ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interferon Regulatory Factor 9

Code

E2760Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.58ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

UniProt accession

MASS(Da)

43,696

GeneID

10379

Synonyms

IFN-alpha-responsive transcription factor subunit;Interferon regulatory factor 9;Interferon-stimulated gene factor 3 gamma;IRF 9;IRF9;IRF-9;ISGF-3 gamma;ISGF3 p48 subunit;Transcriptional regulator ISGF3 subunit gamma;

Gene names

IRF9

Research area

Immunology

Target protein

IRF9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2760Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.2 2.092 2.067
1.984
1000ng/L 1.503 1.454 1.428
1.404
500ng/L 0.542 0.579 0.553
0.616
250ng/L 0.381 0.385 0.359
0.388
125ng/L 0.189 0.191 0.165
0.192
0ng/L 0.023 0.026 0
0.028

Precision

We measured random samples of E2760Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1372.3 54.53 4
Intra-Assay 2 18 479.4 18.52 3.9
Intra-Assay 3 18 689.9 25.88 3.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E2760Hu

    $320.00

  • 96T

    E2760Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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