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Home > Product > ELISA Kit > Human Battenin, BSND ELISA Kit

Human Battenin, BSND ELISA Kit

Human Battenin, BSND ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.64ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.64ng/L

Detection range: 20-3800ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Battenin

Code

E2839Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.64ng/L

Detection range

20-3800ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mediates microtubule-dependent, anterograde transport connecting the Golgi network, endosomes, autophagosomes, lysosomes and plasma membrane, and participates in several cellular processes such as regulation of lysosomal pH, lysosome protein degradation, receptor-mediated endocytosis, autophagy, transport of proteins and lipids from the TGN, apoptosis and synaptic transmission (PubMed: 10924275, PubMed: 18817525, PubMed: 18317235, PubMed: 22261744, PubMed: 15471887, PubMed: 20850431). Facilitates the proteins transport from trans-Golgi network (TGN)-to other membrane compartments such as transport of microdomain-associated proteins to the plasma membrane, IGF2R transport to the lysosome where it regulates the CTSD release leading to regulation of CTSD maturation and thereby APP intracellular processing (PubMed: 10924275, PubMed: 18817525). Moreover regulates CTSD activity in response to osmotic stress (PubMed: 23840424, PubMed: 28390177). Also binds galactosylceramide and transports it from the trans Golgi to the rafts, which may have immediate and downstream effects on cell survival by modulating ceramide synthesis (PubMed: 18317235). At the plasma membrane, regulates actin-dependent events including filopodia formation, cell migration, and pinocytosis through ARF1-CDC42 pathway and also the cytoskeleton organization through interaction with MYH10 and fodrin leading to the regulation of the plasma membrane association of Na+, K+ ATPase complex (PubMed: 20850431). Regulates synaptic transmission in the amygdala, hippocampus, and cerebellum through regulation of synaptic vesicles density and their proximity to active zones leading to modulation of short-term plasticity and age-dependent anxious behavior, learning and memory (By similarity). Regulates autophagic vacuoles (AVs) maturation by modulating the trafficking between endocytic and autophagolysosomal/lysosomal compartments, which involves vesicle fusion leading to regulation of degradation process (By similarity). Participates also in cellular homeostasis of compounds such as, water, ions, amino acids, proteins and lipids in several tissue namely in brain and kidney through regulation of their transport and synthesis (PubMed: 17482562).

UniProt accession

MASS(Da)

47,623

GeneID

1201

Synonyms

Batten disease protein;Battenin;CLN 3;CLN3;Protein CLN3

Gene names

CLN3

Research area

Neuroscience

Target protein

CLN3

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2839Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2000ng/L 2.228 2.202 2.175
2.177
1000ng/L 1.132 1.252 1.225
1.372
500ng/L 0.629 0.636 0.609
0.643
250ng/L 0.429 0.437 0.41
0.445
125ng/L 0.208 0.205 0.177
0.201
0ng/L 0.029 0.027 0
0.026

Precision

We measured random samples of E2839Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1236.3 44.87 3.6
Intra-Assay 2 18 931.9 57.06 6.1
Intra-Assay 3 18 305.2 19.76 6.5

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2839Hu

    $320.00

  • 96T

    E2839Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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