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Home > Product > ELISA Kit > Human Carbohydrate Sulfotransferase 1, CHST1 ELISA Kit

Human Carbohydrate Sulfotransferase 1, CHST1 ELISA Kit

Human Carbohydrate Sulfotransferase 1, CHST1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.021ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carbohydrate Sulfotransferase 1

Code

E2866Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.021ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.

UniProt accession

MASS(Da)

46,715

GeneID

8534

Synonyms

Carbohydrate sulfotransferase 1;CHST 1;CHST1;Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1;GST-1;Keratan sulfate Gal-6 sulfotransferase;KS6ST;KSGal6ST;KSST;

Gene names

CHST1

Research area

Others

Target protein

CHST1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2866Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.081 2.103 2.067
2.126
8ng/ml 1.259 1.245 1.209
1.231
4ng/ml 0.618 0.588 0.552
0.558
2ng/ml 0.338 0.359 0.323
0.38
1ng/ml 0.207 0.194 0.158
0.181
0ng/ml 0.03 0.036 0
0.043

Precision

We measured random samples of E2866Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 7.5 0.36 4.8
Intra-Assay 2 18 11.2 0.42 3.7
Intra-Assay 3 18 4.1 0.23 5.5

Protocols

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  • Size

    Code

    Price

  • 48T

    E2866Hu

    $320.00

  • 96T

    E2866Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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