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Home > Product > ELISA Kit > Human Carbohydrate Sulfotransferase 4, CHST4 ELISA Kit

Human Carbohydrate Sulfotransferase 4, CHST4 ELISA Kit

Human Carbohydrate Sulfotransferase 4, CHST4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carbohydrate Sulfotransferase 4

Code

E2874Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X on receptors SPN/CD43, GLYCAM1 and MADCAM1. Also involved in biosynthesis of SELL ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.

UniProt accession

MASS(Da)

45,134

GeneID

10164

Synonyms

Carbohydrate sulfotransferase 4;CHST 4;CHST4;Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3;GlcNAc6ST-2;Gn6st-2;GST-3;HEC-GlcNAc6ST;High endothelial cells N-acetylglucosamine 6-O-sulfotransferase;L-selectin ligand sulfotransferase;LSST;N-acetylglucosamine 6-O-sulfotransferase 2

Gene names

CHST4

Research area

Others

Target protein

CHST4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2874Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.285 2.118 2.076
1.951
6ng/ml 1.412 1.485 1.443
1.559
3ng/ml 0.719 0.634 0.591
0.548
1.5ng/ml 0.43 0.371 0.329
0.313
0.75ng/ml 0.213 0.185 0.143
0.157
0ng/ml 0.038 0.042 0
0.047

Precision

We measured random samples of E2874Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 2 0.08 3.9
Intra-Assay 2 18 4.2 0.16 3.7
Intra-Assay 3 18 5.4 0.17 3.1

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2874Hu

    $320.00

  • 96T

    E2874Hu

    $458.00

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Lead time: Within two weeks

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