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Home > Product > ELISA Kit > Human Suppressors of Cytokine Signaling 1, SOCS1 ELISA Kit

Human Suppressors of Cytokine Signaling 1, SOCS1 ELISA Kit

Human Suppressors of Cytokine Signaling 1, SOCS1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.102ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.102ng/ml

Detection range: 0.2-60ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Suppressors of Cytokine Signaling 1

Code

E2960Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.102ng/ml

Detection range

0.2-60ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.

UniProt accession

MASS(Da)

23,551

GeneID

8651

Synonyms

JAB;JAK-binding protein;SOCS 1;SOCS1;SOCS-1;SSI-1;STAT-induced STAT inhibitor 1;Suppressor of cytokine signaling 1;Tec-interacting protein 3;TIP-3

Gene names

SOCS1

Research area

Others

Target protein

SOCS1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2960Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.087 2.139 2.108
2.191
20ng/ml 1.589 1.659 1.627
1.728
10ng/ml 0.993 1.05 1.019
1.107
5ng/ml 0.769 0.749 0.718
0.729
2.5ng/ml 0.435 0.446 0.414
0.457
0ng/ml 0.032 0.031 0
0.03

Precision

We measured random samples of E2960Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

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  • Size

    Code

    Price

  • 48T

    E2960Hu

    $320.00

  • 96T

    E2960Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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