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Home > Product > ELISA Kit > Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA Kit

Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA Kit

Human Nuclear Mitotic Apparatus Protein 1, NUMA1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.022ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Nuclear Mitotic Apparatus Protein 1

Code

E2971Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.022ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Microtubule (MT)-binding protein that plays a role in the formation and maintenance of the spindle poles and the alignement and the segregation of chromosomes during mitotic cell division (PubMed: 7769006, PubMed: 17172455, PubMed: 19255246, PubMed: 24996901, PubMed: 26195665, PubMed: 27462074). Functions to tether the minus ends of MTs at the spindle poles, which is critical for the establishment and maintenance of the spindle poles (PubMed: 12445386, PubMed: 11956313). Plays a role in the establishment of the mitotic spindle orientation during metaphase and elongation during anaphase in a dynein-dynactin-dependent manner (PubMed: 23870127, PubMed: 24109598, PubMed: 24996901, PubMed: 26765568). In metaphase, part of a ternary complex composed of GPSM2 and G(i) alpha proteins, that regulates the recruitment and anchorage of the dynein-dynactin complex in the mitotic cell cortex regions situated above the two spindle poles, and hence regulates the correct oritentation of the mitotic spindle (PubMed: 23027904, PubMed: 22327364, PubMed: 23921553). During anaphase, mediates the recruitment and accumulation of the dynein-dynactin complex at the cell membrane of the polar cortical region through direct association with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), and hence participates in the regulation of the spindle elongation and chromosome segregation (PubMed: 22327364, PubMed: 23921553, PubMed: 24996901, PubMed: 24371089). Binds also to other polyanionic phosphoinositides, such as phosphatidylinositol 3-phosphate (PIP), lysophosphatidic acid (LPA) and phosphatidylinositol triphosphate (PIP3), in vitro (PubMed: 24996901, PubMed: 24371089). Also required for proper orientation of the mitotic spindle during asymmetric cell divisions (PubMed: 21816348). Plays a role in mitotic MT aster assembly (PubMed: 11163243, PubMed: 11229403, PubMed: 12445386). Involved in anastral spindle assembly (PubMed: 25657325). Positively regulates TNKS protein localization to spindle poles in mitosis (PubMed: 16076287). Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority of the nuclear volume (PubMed: 10075938). Required for epidermal differentiation and hair follicle morphogenesis (By similarity).

UniProt accession

MASS(Da)

238,260

GeneID

4926

Synonyms

NMP-22;Nuclear matrix protein-22;Nuclear mitotic apparatus protein;Nuclear mitotic apparatus protein 1;NUMA 1;NuMA protein;NUMA1;SP-H antigen

Gene names

NUMA1

Research area

Cell Biology

Target protein

NUMA1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2971Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.408 2.484 2.428
2.56
6ng/ml 1.389 1.359 1.303
1.329
3ng/ml 0.84 0.785 0.729
0.729
1.5ng/ml 0.526 0.473 0.418
0.421
0.75ng/ml 0.243 0.217 0.161
0.191
0ng/ml 0.052 0.056 0
0.06

Precision

We measured random samples of E2971Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 1.7 0.09 5.6
Intra-Assay 2 18 8.4 0.28 3.4
Intra-Assay 3 18 4.4 0.23 5.1

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E2971Hu

    $320.00

  • 96T

    E2971Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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