Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Bovine Major Prion Protein, PRNP ELISA Kit

Bovine Major Prion Protein, PRNP ELISA Kit

Bovine Major Prion Protein, PRNP ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.021ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Major Prion Protein

Code

E2230Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.021ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity).

UniProt accession

MASS(Da)

28,614

GeneID

281427

Synonyms

CD antigen CD230;Major prion protein;Major scrapie-associated fibril protein 1;PRNP;PrP

Gene names

PRNP

Research area

Neuroscience

Target protein

PRNP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2230Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.324 2.183 2.141
2.042
6ng/ml 1.184 1.184 1.142
1.185
3ng/ml 0.86 0.804 0.761
0.747
1.5ng/ml 0.506 0.503 0.461
0.501
0.75ng/ml 0.252 0.255 0.213
0.258
0ng/ml 0.055 0.042 0
0.03

Precision

We measured random samples of E2230Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.9 0.25 3.6
Intra-Assay 2 18 4.3 0.31 7.2
Intra-Assay 3 18 1.8 0.14 7.6

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 96T

    E2230Bo

    $458.00

  • 48T

    E2230Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up