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Home > Product > ELISA Kit > Human Hypoxia-inducible Factor 1-alpha Inhibitor, HIF1AN ELISA Kit

Human Hypoxia-inducible Factor 1-alpha Inhibitor, HIF1AN ELISA Kit

Human Hypoxia-inducible Factor 1-alpha Inhibitor, HIF1AN ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.09ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.09ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Hypoxia-Inducible Factor 1-Alpha Inhibitor

Code

E3080Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.09ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

UniProt accession

MASS(Da)

40,285

GeneID

55662

Synonyms

Factor inhibiting HIF-1;FIH-1;HIF1AN;Hypoxia-inducible factor 1-alpha inhibitor;Hypoxia-inducible factor asparagine hydroxylase

Gene names

HIF1AN

Research area

Others

Target protein

HIF1AN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3080Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.878 1.974 1.924
2.069
600ng/L 1.075 1.03 0.981
0.985
300ng/L 0.431 0.423 0.374
0.415
150ng/L 0.258 0.246 0.197
0.235
75ng/L 0.118 0.129 0.079
0.14
0ng/L 0.044 0.049 0
0.055

Precision

We measured random samples of E3080Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 386.7 14.52 3.8
Intra-Assay 2 18 737.5 18.37 2.5
Intra-Assay 3 18 601.4 23.67 3.9

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3080Hu

    $320.00

  • 96T

    E3080Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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