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Home > Product > ELISA Kit > Human Cytochrome P450 2B6, CYP2B6 ELISA Kit

Human Cytochrome P450 2B6, CYP2B6 ELISA Kit

Human Cytochrome P450 2B6, CYP2B6 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 9.77ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 9.77ng/L

Detection range: 20-4500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 2B6

Code

E3083Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

9.77ng/L

Detection range

20-4500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids (PubMed: 21289075, PubMed: 12865317). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling (PubMed: 21289075). Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2 (PubMed: 21289075, PubMed: 12865317). Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs (PubMed: 11695850, PubMed: 22909231). Acts as a 1,4-cineole 2-exo-monooxygenase (PubMed: 11695850). Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

UniProt accession

MASS(Da)

56,278

GeneID

1555

Synonyms

1,4-cineole 2-exo-monooxygenase;CYP2B6;CYPIIB6;Cytochrome P450 2B6;Cytochrome P450 IIB1

Gene names

CYP2B6

Research area

Others

Target protein

CYP2B6

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3083Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.093 2.047 1.999
2.001
1200ng/L 1.335 1.407 1.36
1.479
600ng/L 0.924 0.927 0.879
0.929
300ng/L 0.41 0.418 0.371
0.427
150ng/L 0.26 0.278 0.231
0.296
0ng/L 0.048 0.047 0
0.046

Precision

We measured random samples of E3083Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 634.7 33.06 5.2
Intra-Assay 2 18 1482.2 89.19 6
Intra-Assay 3 18 314.1 17.86 5.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E3083Hu

    $320.00

  • 96T

    E3083Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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