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Home > Product > ELISA Kit > Human Insulin Receptor Substrate 4, IRS-4 ELISA Kit

Human Insulin Receptor Substrate 4, IRS-4 ELISA Kit

Human Insulin Receptor Substrate 4, IRS-4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.091ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.091ng/ml

Detection range: 0.2-70ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Insulin Receptor Substrate 4

Code

E3128Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.091ng/ml

Detection range

0.2-70ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.

UniProt accession

MASS(Da)

133,768

GeneID

8471

Synonyms

160 kDa phosphotyrosine protein;Insulin receptor substrate 4;IRS 4;IRS4;IRS-4;Phosphoprotein of 160 kDa;pp160;py160

Gene names

IRS4

Research area

Signal Transduction

Target protein

IRS4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3128Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
40ng/ml 2.405 2.32 2.263
2.234
20ng/ml 1.302 1.35 1.294
1.398
10ng/ml 0.873 0.824 0.768
0.775
5ng/ml 0.4 0.455 0.399
0.511
2.5ng/ml 0.256 0.271 0.215
0.285
0ng/ml 0.043 0.056 0
0.07

Precision

We measured random samples of E3128Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 16.5 0.93 5.7
Intra-Assay 2 18 21.7 0.72 3.3
Intra-Assay 3 18 4.8 0.31 6.5

Protocols

Customer reviews and Q&As

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Associated products

  • Size

    Code

    Price

  • 48T

    E3128Hu

    $320.00

  • 96T

    E3128Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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