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Home > Product > ELISA Kit > Human Neurofilament Heavy Polypeptide, NEFH ELISA Kit

Human Neurofilament Heavy Polypeptide, NEFH ELISA Kit

Human Neurofilament Heavy Polypeptide, NEFH ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neurofilament Heavy Polypeptide

Code

E3136Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

UniProt accession

MASS(Da)

112,479

GeneID

4744

Synonyms

200 kDa neurofilament protein;NEFH;Neurofilament heavy polypeptide;Neurofilament triplet H protein;NF-H

Gene names

NEFH

Research area

Neuroscience

Target protein

NEFH

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3136Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.122 2.151 2.099
2.181
40ng/ml 1.883 1.866 1.813
1.848
20ng/ml 1.143 1.139 1.087
1.136
10ng/ml 0.64 0.638 0.586
0.637
5ng/ml 0.423 0.386 0.333
0.349
0ng/ml 0.059 0.053 0
0.047

Precision

We measured random samples of E3136Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 43.1 3.15 7.3
Intra-Assay 2 18 14.5 0.72 5
Intra-Assay 3 18 5.5 0.3 5.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E3136Hu

    $320.00

  • 96T

    E3136Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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