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Home > Product > ELISA Kit > Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Human Acetyl-CoA Carboxylase 1, ACACA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.34ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Acetyl-Coa Carboxylase 1

Code

E3172Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.34ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed: 20952656, PubMed: 20457939, PubMed: 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed: 20952656, PubMed: 20457939, PubMed: 29899443).

UniProt accession

MASS(Da)

265,554

GeneID

31

Synonyms

ACACA;ACC1;ACC-alpha;Acetyl-CoA carboxylase 1;Acetyl-Coenzyme A carboxylase alpha

Gene names

ACACA

Research area

Metabolism

Target protein

ACACA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3172Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 2.071 2.103 2.064
2.135
320ng/L 1.598 1.618 1.579
1.638
160ng/L 1.076 1.098 1.059
1.12
80ng/L 0.708 0.745 0.706
0.783
40ng/L 0.452 0.433 0.394
0.414
0ng/L 0.036 0.039 0
0.042

Precision

We measured random samples of E3172Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 415.7 27.8 6.7
Intra-Assay 2 18 114.2 5.19 4.5
Intra-Assay 3 18 35 2.01 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3172Hu

    $320.00

  • 96T

    E3172Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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