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Home > Product > ELISA Kit > Human Acetyl-CoA Carboxylase 2, ACACB ELISA Kit

Human Acetyl-CoA Carboxylase 2, ACACB ELISA Kit

Human Acetyl-CoA Carboxylase 2, ACACB ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.023ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Acetyl-Coa Carboxylase 2

Code

E3173Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.023ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Mitochondrial enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and plays a central role in fatty acid metabolism (PubMed: 16854592, PubMed: 19236960, PubMed: 20457939, PubMed: 20952656, PubMed: 19900410, PubMed: 26976583). Catalyzes a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed: 19236960, PubMed: 20457939, PubMed: 20952656, PubMed: 26976583). Through the production of malonyl-CoA that allosterically inhibits carnitine palmitoyltransferase 1 at the mitochondria, negatively regulates fatty acid oxidation (By similarity). Together with its cytosolic isozyme ACACA, which is involved in de novo fatty acid biosynthesis, promotes lipid storage (By similarity).

UniProt accession

MASS(Da)

276,541

GeneID

32

Synonyms

ACACB;ACC-beta;Acetyl-CoA carboxylase 2

Gene names

ACACB

Research area

Others

Target protein

ACACB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3173Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
16ng/ml 2.281 2.196 2.154
2.111
8ng/ml 1.604 1.668 1.626
1.732
4ng/ml 1.107 1.149 1.107
1.191
2ng/ml 0.755 0.72 0.678
0.685
1ng/ml 0.468 0.475 0.433
0.482
0ng/ml 0.052 0.042 0
0.033

Precision

We measured random samples of E3173Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E3173Hu

    $320.00

  • 96T

    E3173Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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