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Home > Product > ELISA Kit > Bovine Myeloid Differentiation Primary Response Protein Myd88, MYD88 ELISA Kit

Bovine Myeloid Differentiation Primary Response Protein Myd88, MYD88 ELISA Kit

Bovine Myeloid Differentiation Primary Response Protein Myd88, MYD88 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 8.12ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 8.12ng/L

Detection range: 15-3000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Myeloid Differentiation Primary Response Protein Myd88

Code

E2236Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

8.12ng/L

Detection range

15-3000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes (PubMed: 17936907, PubMed: 18760477). MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine (By similarity).

UniProt accession

MASS(Da)

33,709

GeneID

444881

Synonyms

MYD 88;MYD88;Myeloid differentiation primary response protein MyD88

Gene names

MYD88

Research area

Signal Transduction

Target protein

MYD88

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2236Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1600ng/L 2.012 1.981 1.934
1.95
800ng/L 1.163 1.05 1.003
0.937
400ng/L 0.402 0.412 0.366
0.422
200ng/L 0.252 0.228 0.181
0.204
100ng/L 0.131 0.13 0.084
0.13
0ng/L 0.062 0.047 0
0.032

Precision

We measured random samples of E2236Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 972.5 24.49 2.5
Intra-Assay 2 18 333.6 11.39 3.4
Intra-Assay 3 18 612.4 16.97 2.8

Protocols

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Associated products

  • Size

    Code

    Price

  • 96T

    E2236Bo

    $458.00

  • 48T

    E2236Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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