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Home > Product > ELISA Kit > Human Tubulin Polymerization-promoting Protein, TPPP ELISA Kit

Human Tubulin Polymerization-promoting Protein, TPPP ELISA Kit

Human Tubulin Polymerization-promoting Protein, TPPP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.017ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tubulin Polymerization-Promoting Protein

Code

E3198Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.017ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Regulator of microtubule dynamics that plays a key role in myelination by promoting elongation of the myelin sheath (PubMed: 31522887). Acts as a microtubule nucleation factor in oligodendrocytes: specifically localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, and promotes microtubule nucleation, an important step for elongation of the myelin sheath (PubMed: 31522887). Required for both uniform polarized growth of distal microtubules as well as directing the branching of proximal processes (PubMed: 31522887). Shows magnesium-dependent GTPase activity; the role of the GTPase activity is unclear (PubMed: 21995432, PubMed: 21316364). In addition to microtubule nucleation activity, also involved in microtubule bundling and stabilization of existing microtubules, thereby maintaining the integrity of the microtubule network (PubMed: 17105200, PubMed: 17693641, PubMed: 18028908, PubMed: 26289831). Regulates microtubule dynamics by promoting tubulin acetylation: acts by inhibiting the tubulin deacetylase activity of HDAC6 (PubMed: 20308065, PubMed: 23093407). Also regulates cell migration: phosphorylation by ROCK1 inhibits interaction with HDAC6, resulting in decreased acetylation of tubulin and increased cell motility (PubMed: 23093407). Plays a role in cell proliferation by regulating the G1/S-phase transition (PubMed: 23355470). Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis; this process is regulated by phosphorylation by LIMK2 (PubMed: 22328514).

UniProt accession

MASS(Da)

23,694

GeneID

11076

Synonyms

25 kDa brain-specific protein;p24;p25-alpha;TPPP;TPPP/p25;Tubulin polymerization-promoting protein

Gene names

TPPP

Research area

Signal Transduction

Target protein

TPPP

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3198Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.203 2.133 2.092
2.064
6ng/ml 1.405 1.492 1.451
1.579
3ng/ml 0.604 0.598 0.556
0.592
1.5ng/ml 0.32 0.328 0.286
0.336
0.75ng/ml 0.128 0.122 0.08
0.116
0ng/ml 0.054 0.041 0
0.028

Precision

We measured random samples of E3198Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 5.4 0.12 2.2
Intra-Assay 2 18 3.3 0.09 2.8
Intra-Assay 3 18 2.6 0.08 3

Protocols

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  • Size

    Code

    Price

  • 48T

    E3198Hu

    $320.00

  • 96T

    E3198Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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