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Home > Product > ELISA Kit > Bovine Protein Disulfide-isomerase, P4HB ELISA Kit

Bovine Protein Disulfide-isomerase, P4HB ELISA Kit

Bovine Protein Disulfide-isomerase, P4HB ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.32ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.32ng/mL

Detection range: 0.5-200ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Protein Disulfide-Isomerase

Code

E2246Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.32ng/mL

Detection range

0.5-200ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

UniProt accession

MASS(Da)

57,266

GeneID

281373

Synonyms

Cellular thyroid hormone-binding protein;P4HB;p55;PDI;Prolyl 4-hydroxylase subunit beta;Protein disulfide-isomerase

Gene names

P4HB

Research area

Others

Target protein

P4HB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2246Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
120ng/mL 1.707 1.755 1.697
1.803
60ng/mL 0.929 0.902 0.844
0.875
30ng/mL 0.425 0.45 0.392
0.476
15ng/mL 0.213 0.242 0.183
0.27
7.5ng/mL 0.092 0.115 0.056
0.137
0ng/mL 0.061 0.058 0
0.055

Precision

We measured random samples of E2246Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 77.7 4.19 5.4
Intra-Assay 2 18 113.7 2.5 2.2
Intra-Assay 3 18 32 1.53 4.8

Protocols

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  • Size

    Code

    Price

  • 96T

    E2246Bo

    $458.00

  • 48T

    E2246Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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