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Home > Product > ELISA Kit > Canine Intercellular Adhesion Molecule 1, ICAM-1 ELISA Kit

Canine Intercellular Adhesion Molecule 1, ICAM-1 ELISA Kit

Canine Intercellular Adhesion Molecule 1, ICAM-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.55ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product Summary

Size: 48T,96T

Sensitivity: 3.55ng/ml

Detection range: 7-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Canine

Product overview

Full product name

Intercellular adhesion molecule 1

Code

E0017Ca

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.55ng/ml

Detection range

7-1500ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Canine

Storage

2-8ºC

Assay time

1h 30m

Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

UniProt accession

MASS(Da)

57,952

GeneID

403975

Synonyms

CD antigen CD54;Fragment;ICAM-1;Intercellular adhesion molecule 1

Gene names

ICAM1

Research area

Others

Target protein

ICAM-1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0017Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/ml 2.229 2.304 2.249
2.379
400ng/ml 1.844 1.841 1.785
1.838
200ng/ml 1.119 1.1 1.044
1.08
100ng/ml 0.702 0.686 0.63
0.67
50ng/ml 0.361 0.399 0.343
0.436
0ng/ml 0.058 0.056 0
0.053

Precision

We measured random samples of E0017Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 177.64 11.87 6.68
Intra-Assay 2 18 291.65 11.45 3.92
Intra-Assay 3 18 54.25 3.14 5.79

Protocols

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  • Size

    Code

    Price

  • 48T

    E0017Ca

    $350.00

  • 96T

    E0017Ca

    $475.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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