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Home > Product > ELISA Kit > Human Neurogenic Locus Notch Homolog Protein 1, NOTCH1 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 1, NOTCH1 ELISA Kit

Human Neurogenic Locus Notch Homolog Protein 1, NOTCH1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.27ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neurogenic Locus Notch Homolog Protein 1

Code

E3218Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.27ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

UniProt accession

MASS(Da)

272,505

GeneID

4851

Synonyms

hN1;Neurogenic locus notch homolog protein 1;NEXT;NICD;NOTCH 1;Notch 1 extracellular truncation;Notch 1 intracellular domain;NOTCH1;Translocation-associated notch protein TAN-1;

Gene names

NOTCH1

Research area

Epigenetics and Nuclear Signaling

Target protein

NOTCH1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3218Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
96ng/ml 2.172 2.295 2.227
2.418
48ng/ml 1.424 1.428 1.36
1.431
24ng/ml 0.735 0.721 0.653
0.706
12ng/ml 0.433 0.464 0.396
0.494
6ng/ml 0.312 0.28 0.212
0.248
0ng/ml 0.062 0.068 0
0.074

Precision

We measured random samples of E3218Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 54.4 2.43 4.5
Intra-Assay 2 18 12.8 0.78 6.1
Intra-Assay 3 18 41.3 2.38 5.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3218Hu

    $320.00

  • 96T

    E3218Hu

    $458.00

Add to Cart

Lead time: Within two weeks

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