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Home > Product > ELISA Kit > Human Cytochrome P450 1A1, CYP1A1 ELISA Kit

Human Cytochrome P450 1A1, CYP1A1 ELISA Kit

Human Cytochrome P450 1A1, CYP1A1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.25ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.25ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cytochrome P450 1A1

Code

E3222Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.25ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301, PubMed: 15041462, PubMed: 18577768, PubMed: 19965576, PubMed: 20972997, PubMed: 10681376). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C15-alpha and C16-alpha positions (PubMed: 11555828, PubMed: 14559847, PubMed: 12865317, PubMed: 15805301). Displays different regioselectivities for polyunsaturated fatty acids (PUFA) hydroxylation (PubMed: 15041462, PubMed: 18577768). Catalyzes the epoxidation of double bonds of certain PUFA (PubMed: 15041462, PubMed: 19965576, PubMed: 20972997). Converts arachidonic acid toward epoxyeicosatrienoic acid (EET) regioisomers, 8,9-, 11,12-, and 14,15-EET, that function as lipid mediators in the vascular system (PubMed: 20972997). Displays an absolute stereoselectivity in the epoxidation of eicosapentaenoic acid (EPA) producing the 17(R),18(S) enantiomer (PubMed: 15041462). May play an important role in all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed: 10681376). May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH-independent) (PubMed: 21068195).

UniProt accession

MASS(Da)

58,165

GeneID

1543

Synonyms

CYP 1A1;CYP1A1;CYPIA1;Cytochrome P450 1A1;Cytochrome P450 form 6;Cytochrome P450-C;Cytochrome P450-P1;Hydroperoxy icosatetraenoate dehydratase

Gene names

CYP1A1

Research area

Cardiovascular

Target protein

CYP1A1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3222Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 1.944 1.972 1.931
1.999
600ng/L 1.336 1.423 1.383
1.511
300ng/L 0.559 0.559 0.518
0.558
150ng/L 0.45 0.42 0.379
0.39
75ng/L 0.207 0.162 0.122
0.118
0ng/L 0.042 0.041 0
0.039

Precision

We measured random samples of E3222Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 216.9 8.34 3.8
Intra-Assay 2 18 342.9 14.93 4.4
Intra-Assay 3 18 879.2 46.79 5.3

Protocols

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  • Size

    Code

    Price

  • 48T

    E3222Hu

    $320.00

  • 96T

    E3222Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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