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Home > Product > ELISA Kit > Human Fructosamine-3-kinase, FN3K ELISA Kit

Human Fructosamine-3-kinase, FN3K ELISA Kit

Human Fructosamine-3-kinase, FN3K ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Fructosamine-3-Kinase

Code

E3233Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.054ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed: 11016445, PubMed: 11522682, PubMed: 11975663). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed: 11522682, PubMed: 11975663). Involved in intracellular deglycation in erythrocytes (PubMed: 11975663). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (By similarity). Also able to phosphorylate psicosamines and ribulosamines (PubMed: 14633848).

UniProt accession

MASS(Da)

35,171

GeneID

64122

Synonyms

FN 3K;FN3K;Fructosamine-3-kinase;Protein-psicosamine 3-kinase FN3K;Protein-ribulosamine 3-kinase FN3K

Gene names

FN3K

Research area

Signal Transduction

Target protein

FN3K

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3233Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 1.938 1.888 1.848
1.839
12ng/ml 1.053 0.987 0.947
0.922
6ng/ml 0.524 0.5 0.459
0.476
3ng/ml 0.22 0.253 0.212
0.285
1.5ng/ml 0.143 0.138 0.097
0.133
0ng/ml 0.035 0.041 0
0.047

Precision

We measured random samples of E3233Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.4 0.48 3.1
Intra-Assay 2 18 11.6 0.42 3.7
Intra-Assay 3 18 4.5 0.22 4.8

Protocols

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  • Size

    Code

    Price

  • 48T

    E3233Hu

    $320.00

  • 96T

    E3233Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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