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Home > Product > ELISA Kit > Human Rho Guanine Nucleotide Exchange Factor 1, ARHGEF1 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 1, ARHGEF1 ELISA Kit

Human Rho Guanine Nucleotide Exchange Factor 1, ARHGEF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.79ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 2.79ng/L

Detection range: 5-1000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Rho Guanine Nucleotide Exchange Factor 1

Code

E3234Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.79ng/L

Detection range

5-1000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits (PubMed: 9641915, PubMed: 9641916). Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase (PubMed: 9641915, PubMed: 9641916, PubMed: 8810315, PubMed: 30521495). Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain (PubMed: 9641916). This GEF activity is inhibited by binding to activated GNA12 (PubMed: 9641916). Mediates angiotensin-2-induced RhoA activation (PubMed: 20098430).

UniProt accession

MASS(Da)

102,435

GeneID

9138

Synonyms

115 kDa guanine nucleotide exchange factor;ARHGEF 1;ARHGEF1;p115RhoGEF;p115-RhoGEF;Rho guanine nucleotide exchange factor 1;Sub1.5

Gene names

ARHGEF1

Research area

Others

Target protein

ARHGEF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3234Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/L 1.844 1.88 1.841
1.916
320ng/L 0.942 1.058 1.018
1.173
160ng/L 0.528 0.494 0.455
0.461
80ng/L 0.163 0.239 0.2
0.316
40ng/L 0.164 0.12 0.08
0.075
0ng/L 0.025 0.039 0
0.054

Precision

We measured random samples of E3234Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 131.3 5.1 3.9
Intra-Assay 2 18 345.7 17.62 5.1
Intra-Assay 3 18 384.7 10.25 2.7

Protocols

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Associated products

  • Size

    Code

    Price

  • 48T

    E3234Hu

    $320.00

  • 96T

    E3234Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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